Fig. 3.
Effects of varying concentrations of (−)-rhazinilam on assembly in glutamate with 10 μM GTP. Each reaction mixture contained 10 μM tubulin, 0.75 M monosodium glutamate, 10 μM GTP, 4% dimethyl sulfoxide, and concentrations of (−)-rhazinilam as follows: curve 1, none; curve 2, 2 μM; curve 3, 5 μM; curve 4, 10 μM; curve 5, 20 μM. Incubation was at 30 °C.