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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Jun 2;487(2):98–104. doi: 10.1016/j.abb.2009.05.014

Fig. 5.

Fig. 5

Effect of 20 μM (−)-rhazinilam on assembly in glutamate without GTP and with 0.4 mM GTP. Each reaction mixture contained 10 μM tubulin, 0.75 M monosodium glutamate, 4% dimethyl sulfoxide, and concentrations of (−)-rhazinilam and GTP as follows: curve 1, no GTP and no (−)-rhazinilam; curve 2, no GTP and 20 μM (−)-rhazinilam; curve 3, 0.4 mM GTP and no (−)-rhazinilam; curve 4, 0.4 mM GTP and 20 μM (−)-rhazinilam. At zero time the temperature controller was set at 30 °C. At the time indicated by the vertical dashed line, the temperature controller was set at 0 °C.