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. 2009 Jun 25;28(15):2259–2271. doi: 10.1038/emboj.2009.170

Figure 2.

Figure 2

RGL2, GAI and RGA are necessary to promote endogenous ABA and ABI5 levels under FR conditions. (A) Time course of endogenous ABA levels in ga1-3, ga1-3/rgl2-13 and ga1-3/rga-t2/gai-t6/rgl2-1 seeds under white light conditions (normal MS medium). Units are pmol per gram of fresh weight. Error bars indicate s.d. (n=3). (B) Same as in (A) under far-red light conditions. (C) Protein gel blot analysis of a time course of ABI5 protein levels upon ga1-3, ga1-3/rgl2-13 and ga1-3/rga-t2/gai-t6/rgl2-1 seed imbibition under far-red (FR) or white (white) light conditions. Signals can be directly compared between different genetic backgrounds. Each lane contains proteins extracted from 5 mg of seeds stained with Ponceau S (Ponceau) before incubation with antibodies against ABI5. At each time point, the percentage of germination (i.e. endosperm rupture events) in the seed population material used in the protein blot is indicated. We measured endosperm rupture in three different seed batches (n=150–300): at 5 days endosperm rupture percentage is either 0 or 100% depending on the genotype and the light condition as shown in Figure 1.