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. 2009 Jun 25;28(15):2259–2271. doi: 10.1038/emboj.2009.170

Figure 3.

Figure 3

ABA blocks rupture of endosperm in ga1/rga/gai/rgl2 seeds, but not that of testa. (A) Representative pictures show testa rupture events in ga1-3 and ga1-3/rga-t2/gai-t6/rgl2-1 seeds 5 days after imbibition under white (white) or far-red (FR) conditions in the presence of 3 μM ABA. Histogram shows percentage of testa and endosperm rupture events 5 days after seed imbibition. Arrows indicate testa rupture event (in three independent seed batches (n=150–300) testa rupture percentage is always either 0 or 100% depending on the genotype and the light conditions as shown in the histogram). (B) Protein gel blot analysis of ABI5 protein levels in ga1-3 and ga1-3/rga-t2/gai-t6sol;rgl2-1 seeds at the indicated times upon imbibition under far-red (FR) or white (W) light conditions in the presence of 3 μM ABA. Protein gel blot conditions as in Figure 2C.