Figure 8.

ABA acts through ABI3 to inhibit endosperm rupture in FR conditions. Pictures show WT (Col), pil5, WT (Ler), aba1-1 and abi3-1 seeds 7 days upon imbibition under white (white) or far-red (FR) conditions in the presence of 5 μM PAC (PAC) (concerning the use of PAC, see Supplementary Figure 10). Seeds were used for puncture experiments (Materials and methods). Punctured seeds were transferred to normal medium (MS) under white light conditions to assess seed viability after surgical procedure (Supplementary Figure 6). The procedure did not prevent seed germination (Supplementary Figure 6 shows WT (Col and Ler) and pil5 seeds after puncture).