Skip to main content
. 2009 Jun 25;28(15):2259–2271. doi: 10.1038/emboj.2009.170

Figure 9.

Figure 9

aba1 and abi3 seeds accumulate WT-like levels of GAI and RGA consistent with their inability to rupture the testa. DELLA-dependent increase in endogenous ABA levels correlates with high ABI3 mRNA expression. (A) Protein gel blot analysis of RGA and GAI protein levels in WT (Ler) and aba1-1 and abi3-1 seeds at the indicated times upon seed imbibition under far-red conditions in the presence of 5 μM PAC (FR+PAC) (concerning the use of PAC, see Supplementary Figure 10); 10 μg of total protein is loaded per lane. (B) RNA gel blot analysis of the time course of ABI3 mRNA accumulation in ga1-3, ga1-3/rgl2-13 and ga1-3/rga-t2/gai-t6/rgl2-1 seeds under white or far-red light conditions (normal MS medium). Two micrograms of total RNA was used per lane. rRNA is shown as a loading control. At each time point, the percentage of germination (i.e. endosperm rupture events) in the seed population material used in the RNA blot is indicated. (C) RNA gel blot analysis of the time course of ABI3 mRNA accumulation in PAC-treated (15 μM) WT (Col), pro-RGL2∷GAI, pro-RGL2∷RGA transgenic lines and rgl2-13 seeds under white light conditions. Two micrograms of total RNA was used per lane. rRNA is shown as a loading control. At each time point, the percentage of germination (i.e. endosperm rupture events) in the seed population material used in the RNA blot is indicated.