Figure 4.

Interaction of UPF2 with the CH-domain of UPF1 studied using NMR. Overlay of ¹H, ¹⁵N HSQC spectra of the ¹⁵N-labelled UPF1 CH domain (residues 115–287), free (green) and in complex with unlabelled UPF2 comprising (A) the complete bipartite binding motif (residues 1105–1207), (B) the helical motif (residues 1105–1129) and (C) the β-hairpin motif (residues 1167–1207) (blue). The corresponding complexes are indicated schematically. Assigned chemical-shift perturbations are mapped onto the surface of UPF1 and are coloured in red. Cyan residues could not be analysed because of a lack of NMR signal.