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. 2009 Jun 18;28(15):2283–2292. doi: 10.1038/emboj.2009.171

Figure 3.

Figure 3

Location of the intron and the 5′ end of the pre-mRNA within the head domain of the B complex. B complexes were assembled on a pre-mRNA with MS2–MBP protein bound to MS2 hairpins positioned at either the 5′ end of the 5′ exon (A, B) or within the intron (C, D). After incubation with or without α-MBP and a second centrifugation step, gold-tagged Protein A was added. EM fields (A, C) with (upper field) or without (lower field) α-MBP are shown, as well as selected images (B, D). Illustrative drawings (B, D, on the right) are provided, in which the α-MBP binding-site area determined using the 17-nm criterion (see text) is shaded.