Figure 6.
mRNA and protein expression of mitochondrial biogenesis factors in the cortex. A, Representative agarose gel of RT-PCR products of PGC-1, NRF-1, and TFAM mRNA prepared from control (C) or at the indicated times after H-I. The histogram below shows semiquantitative measurement of PCR products from (A) obtained by densitometric analysis relative to the control level. B, Analysis of quantitative real-time RT-PCR from control or H-I brains at the indicated times for PGC-1, NRF-1, and TFAM normalized to β-actin. C, Representative Western blots for PGC-1 and NRF-1 in cortex from control and various time points after H-I. For each group in all experiments, n=6 pups. *P<0.05 compared with the respective control.