(A) Schematic representation of the Seryl-tRNA synthetase protein and the location of the lesions in the two mutant alleles Sarss277 and Sarss228. The blue bar outlines a highly homologous area containing the serine-recognition site, the red bar the conserved enzymatic core, and the green bar the vertebrate-specific C-terminal appendage.
(B-D) Cycloheximide (cyc) treatment of zebrafish embryos between 60 and 72hpf; epifluorescence micrographs of DMSO-control (B), 100μM cyc (C) and 200μM cyc (D) treated larvae visualized for Tg(flk1:EGFP)s843 expression.