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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Neurochem. 2009 Jun 15;110(4):1226–1240. doi: 10.1111/j.1471-4159.2009.06212.x

Fig. 1. Post pMCAO-insult treatment of rats with SB increased the number of BrdU (+) cells in the SVZ and DG of the ischemic hemisphere.

Fig. 1

Brain tissues were analyzed by double immunostaining with BrdU (red) and NeuN (green, a mature neuronal marker).

(A & B) Seven days post pMCAO treatment. (a) Sham-operated, (b) vehicle-treated, pMCAO, (c) SB-treated, pMCAO, (d) TSA-treated, pMCAO. Arrows identify BrdU (+) cells. Scale bar = 50 µm. The vehicles for SB and TSA were saline and DMSO respectively; no significant differences were found in stimulating cell proliferation.

(C, D & E) Representative data analyzed from 3–4 animals per group, 14 days post pMCAO treatment. (a) Sham, (b) vehicle, (c) SB. Arrows identify BrdU (+) cells. Scale bar = 50 µm. Fewer BrdU (+) cells were found in the contralateral hemisphere than in the ipsilateral hemisphere (E).

(F & G) Quantified results of the number of BrdU (+) cells in the ipsilateral SVZ on day 7, and DG on Day 14 post-pMCAO, respectively. Data are mean ± SEM (n = 3–4 animals per group). **p<0.01, ***p<0.001, between indicated groups. SVZ: subventricular zone, LV: lateral ventricle. DG: dentate gyrus, H: hilus, SGZ: subgranular zone, GCL: granular cell layer.