A. Representative examples of 4 div hippocampal neurons transfected with a shRNA control sequence (left) or shRNA interfering sequence to knockdown endogenous Mecp2 (right). Neurons were co-transfected with eGFP (green) to perform quantitative morphological analyses (scale bar = 10μm). B. Population data on axonal length and branch points. C. Population data on dendritic length and branch points. In this and all subsequent figures, * indicates p<0.05, ** indicates p<0.01, and *** indicates p<0.001, from unpaired Student's t test or one-way ANOVA (see text for details). Additionally, in this and all subsequent figures, arrowheads indicate the position of the axons.