Fig. 1. Effect of MeHg, CdCl2and HgCl2on IL-6 release and cellular viability in primary mouse glia.
Mouse glia were treated with MeHg, CdCl2 or HgCl2 overnight (>18 hrs), and then the medium in each well was collected for IL-6 ELISA and cellular viability was determined by the MTT viability assay. Results indicated that MeHg (Fig. 1A) caused concentration-dependent decrease of viability (open circles, left-side axis) and concurrent increase of IL-6 release (solid circles, right-side axis). Both CdCl2 (Fig. 1B) and HgCl2 (Fig. 1C) caused concentration-dependent decrease of viability (open circles, left-side axis). However, these two agents did not induce IL-6 release (solid circles, right-side axis).