Figure 2.

In vitro zinc addition influences the effect of activation on IFN-γ, MT, and IL-2 expression in primary T cells. Relative mRNA levels of IFN-γ (A) and MT and IL-2 (B) were measured by qPCR in nonactivated and activated cells. The cells were activated after an initial 2-h incubation with the indicated concentration of zinc. Data are normalized to 18S rRNA and are expressed as relative to the nonactivated control that was adjusted to one. Representative data from three independent experiments are presented. Values shown are means ± sd (n=3–4). A different superscript indicates the means are statistically different (P < 0.05–0.001).