Figure 4.

Decrease in intracellular-free zinc in activated primary T cells. FluoZin-3 fluoresence was used to assess labile zinc changes in nonactivated and activated cells. Flow cytometry experiments were conducted in the absence or presence of 100 μM ZnCl₂, which was added for 1.5 min (A). Fluorescence intensity (green) was examined by flow cytometry. T cells were activated for 48 h and probed with MT antibody (blue). Images from nonactivated and activated cells were obtained by laser-scanning confocal microscopy. (B) The area in white boxes represents magnified images. Confocal microscopy was used to visualize localization of FluoZin-3 and lysosomes (C). After initial incubation with FluoZin-3, nonactivated T cells were probed with LysoTracker (red), and colocalization (yellow) with free zinc was shown in merged images.