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. 2009 May 11;86(2):303–312. doi: 10.1189/jlb.1008587

Figure 1.

Figure 1.

The cytokine-inducing capacities of air pollution particles NIST IRM PM2.5 and NIST SRM 1648. Peritoneal macrophages obtained from control (TLR4+/−) mice were plated in 12-well plates (1×106 cells per well) and treated for 24 h with medium, ambient air particles NIST IRM PM2.5 (PM2.5), NIST SRM 1648 (PM10) (125 μg/ml each), 1 μg/ml Pam3Cys, and 100 ng/ml LPS. Following stimulation, supernatants were collected to analyze secretion levels of TNF-α and RANTES by ELISA. Data are presented as mean ± sd from 5 independent experiments. *, P < 0.05 compared with medium-treated cells.