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rhHx suppresses LPS-induced TNF and IL-6 from macrophages. BMDMs (A and B) or peritoneal macrophages (C and D) were incubated overnight with 20 ng/ml LPS and the indicated concentrations (A and B) or 40 μg/ml (C and D) mHx and rhHx. Concentrations of TNF and IL-6 in the supernatants were determined by ELISA. The results represent mean ± se and are representative of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001, compared with cells treated with PBS.
