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Effects of Hx on cytokine production from macrophages stimulated by Pam3Cys. BMDMs were incubated overnight with mHx (100 μg/ml) or PBS with Pam3Cys (200 ng/ml). Concentrations of TNF and IL-6 in the supernatants were determined by ELISA. The results represent mean ± se and are representative of more than five independent experiments. *, P < 0.05, compared with cells treated with PBS.
