Figure 2.

Quantification of human lymphocytes in thymus and spleen. (A) Isolated thymocytes were stained for anti-hCD4 and anti-hCD8 to characterize the distribution of single-positive, double-positive, and double-negative cell populations (images are representative of three mice/group). (B) Spleens were harvested at 8 weeks or 20 weeks postadoptive transfer, respectively. Splenocytes were stained for the human surface markers CD4, CD8, and CD19, and distribution was determined. One representative flow cytometry figure is shown for each stain. Table 1 indicates the number of investigated animals, the average percentage of positive cells for each stain, as well as the maximum and minimum percentage detected.