Figure 6.

Human pro- and anti-inflammatory cytokines are produced in response to sepsis. Mice that had adoptive transfer of hCD34⁺ hematopoietic stem cells 8–12 or 20 weeks previously underwent sham or CLP surgery. Twenty-four hours postsurgery, serum was obtained, and hIFN-γ, hTNF-α, hIL-10, and hIL-6 were determined via BD FACSArray. IL-8 and IL-1β were analyzed only at 20 weeks post-transfer. Serum cytokines in sham-operated mice did not differ at 8–12 weeks versus 20 weeks, and therefore, data were pooled. Note the significant increases in pro- and anti-inflammatory human cytokines in septic humanized mice. Also note the increase in cytokines at 8–12 weeks versus 20 weeks.