Fig. 6.

Protein expression of Notch and Numb in MCF10A and MCF7. a Expression of Notch-1-NECD was not different between the normal breast cell line MCF10A and breast cancer cell line MCF7, but MCF7 cells expressed Notch-ER-precursor stronger than MCF10A cells. b The 14-3-3-ζ vector transfected MCF10A cells protected degradation of Numb-S but the 14-3-3-ζ transfected MCF7 cells did not. The band of 14-3-3-ζ HA-tag indicates expression of transfected 14-3-3-ζ protein. Cells which were transfected vector control have the band of 14-3-3-ζ protein by nature but not the band of 14-3-3-ζ HA tag