Figure 4.
Functional defects in eve meso− larvae. A through C, One segment is shown from the following genotypes of a dissected third instar larval heart stained with neutral red to label PC cells: A, yw: wild-type larvae contain 7 to 8 PCs per segment, on average; B and C, J49 and J43 meso−, respectively (see Figure 1A for transgene diagrams); only 3 PC cells are visible. D, Graph of the average number of PC cells per segment in larvae of the indicated genotypes. E, Graph of average heart rates (in beats/second) of white pre-pupae of the indicated genotypes. D and E, “J49/CyO” is heterozygous for the J49 eve meso− chromosome and the CyO balancer, which is wild-type for the eve gene. All other genotypes (except w and yw, which are considered wild-type) are eve null mutants, rescued with the indicated eve transgene. The number of segments counted (3 to 5 per dissected larva) is shown above each bar. Error bars represent standard deviation. Asterisk indicates significantly different from yw Student’s t test, P<0.01).