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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Anal Chem. 2008 Oct 22;80(22):8656–8661. doi: 10.1021/ac8015938

Figure 1.

Figure 1

Multitarget bacterial cell sorting procedure using the MT-DACS device. (A) The experimental scheme. Step a: target cells (target A and target B) are labeled with DEP tags (tag A and tag B, respectively) via their respective surface markers. Step b: cells are dielectrophoretically sorted and eluted through independent outlets. Step c: collected cells from the outlets are analyzed via flow cytometry to quantify the sorting performance. (B) The physics of multitarget separation via MT-DACS. Two sets of electrodes are positioned at different glancing angles (θ1 and θ2) to select for two different targets. Target A cells labeled with tag A are selected at electrode set A (θ1 = 10°) and elute through outlet A. Target B cells labeled with tag B are sorted at electrode set B (θ2 = 8°) and elute through outlet B. The unlabeled, nontarget cells are not deflected by either electrode set, and they are eluted through the waste outlet.