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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Anal Chem. 2008 Oct 22;80(22):8656–8661. doi: 10.1021/ac8015938

Figure 3.

Figure 3

Experimental setup and optical micrographs of MT-DACS operation. (A) The MT-DACS chip is placed beneath an epifluorescence microscope with two dual-track programmable syringe pumps delivering the sample mixture and buffer fluid. The electrodes are connected to a function generator, and the frequency and magnitude of the applied voltage are measured by a digital oscilloscope. The flow pattern during the separation is monitored by a high-speed camera. (B) Overlaid optical micrographs show tag A, tag B, and nontarget beads being eluted via outlet A, outlet B, and the waste outlet, respectively. Sinusoidal voltage of 20 Vpeak to peak was applied to both sets of electrodes at 500 kHz, and the device was operated at a throughput of ~1.5 × 107 particles/h.