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. 2009 Jul 31;212(16):2571–2578. doi: 10.1242/jeb.030221

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Fig. 2. — Effects of serotonin on the pH_i in isolated and perfused anterior or posterior midguts. (A) pH_i time courses of representative areas of interest from the anterior (red) and posterior (black) midgut of larval (4th instar) Aedes aegypti, showing the influence of addition of 0.2 μmol l^–1 of serotonin (arrow) to the hemolymph-side bath. Hemolymph-side bath: oxygenated mosquito saline (pH 7). Luminal perfusate: NaCl (100 mmol l^–1, pH 7). (B) Average pH_i values for anterior (N=71 areas of interest from 10 different tissues) and posterior (N=19 areas of interest from four different tissues) midguts before and after addition of serotonin. Asterisk indicates statistically significant difference from control (P<0.05).