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. 2009 Jul 31;212(16):2571–2578. doi: 10.1242/jeb.030221

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Fig. 3. — Influence of luminal pH (pH_L) 10 on pH_i in the anterior midgut. (A) pH_i time course of a representative area of interest from the anterior midgut of larval (4th instar) Aedes aegypti, showing the influence of hemolymph-side addition of serotonin (0.2 μmol l^–1) in the presence of hemolymph-side mosquito saline (pH 7) and luminal NaCl (100 mmol l^–1, pH 7), and the effect of an increase of the pH_L to 10. (B) Average pH_i for anterior midguts (N=41 areas of interest of four different tissues) under control conditions, after hemolymph-side addition of serotonin, and after switching to a luminal perfusate of pH 10. Asterisk indicates statistically significant difference from control plus serotonin (P<0.05).