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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Oncogene. 2009 Jun 22;28(32):2873–2881. doi: 10.1038/onc.2009.153

Figure 4. Effect of E2F3 and YY1 knockdown on global gene expression.

Figure 4

A. Western blot analysis using cell extracts from asynchronously growing HEK293 cells with untransfected as a control, or transfected with effective YY1 RNAi duplexes, or transfected with effective E2F3 RNAi duplexes. Protein levels were detected by Western blotting using antibodies against YY1 or E2F3, respectively. Levels of tubulin protein were detected by Western blotting using an antibody against α-tubulin as a control.

B. Effect of E2F3 and YY1 knockdown on gene expression. Microarray analysis was done to identify genes exhibiting changes in regulation in response to transfection of siRNAs targeting YY1 or E2F3. Fold changes in expression of the twenty genes most effected in E2F3 knockdowns relative to untransfected cells are given (fold changes in expression of the same genes in YY1 knockdown cells relative to untransfected cells are also given). Asterisks indicate genes examined in Figure 4C.

C. Asynchronously growing HEK293 cells were harvested and endogenous ChIP experiments were done as described in Materials and Methods. Promoters assayed and antibodies used in the endogenous ChIP experiments are as indicated, NR=normal rabbit.