Fig. 3.

Quantitative analysis of ipsilateral and contralateral microgliosis in L5 lumbar spinal cord tissue from: naïve, sham operated, nerve injured (L5 SNT), and L5 SNT nerve injured animals treated with minocycline (40 mg/kg) at postoperative day 7. (A) Qualitative rating of microgliosis revealed by immunohistochemisty using a 4 point scale raging from 0 (no microgliosis) to 3 (pronounced microgliosis) according to levels of OX42-IR and microglia morphology. Results expressed as mean rating score ± S.E.M. of 8–11 spinal cord sections. (B) Quantitative flow cytometry analysis of total OX42/ED9 positive cells per mg tissue in L5 lumbar spinal cord tissue. Results expressed as mean ± S.E.M. (n = 4/group). Differences between naïve and treated groups ipsilateral spinal cord, *p > 0.05, **p > 0.01. Differences between ipsilateral and contralateral spinal cord tissue within each experimental group, ^†p > 0.05.