Table 3.

Correlation between qualitative reference method and semi-quantitative image analysis paradigms

Method	CY3	DAB
GSI i	0.257	−0.535
GSI ii	0.091	−0.713*
GM i Area %	0.844*	0.859*
GM ii Intercept H	0.561	0.853*
GM iii Intercept V	0.564	0.862*
GM iv Count	0.603	0.614
GM v Count/area	0.452	−0.920*

Correlations based on comparison of microgliosis measured from ipsilateral L5 SNT spinal cord tissue in which microglial cells were identified by OX42-IR, using either fluorescent (CY3) or biotinylated (DAB) secondary visualisation. GSI, grey scale intensity measure (i, conventional grey scale; ii, expanded grey scale); GM, grid measurements. The Pearson correlation was used in correlating the semi-quantitative methods to the qualitative reference method. *Denotes significant correlations (p < 0.05).