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. Author manuscript; available in PMC: 2010 Aug 7.
Published in final edited form as: Cell Stem Cell. 2009 Aug 7;5(2):204–213. doi: 10.1016/j.stem.2009.06.002

Figure 3. Protein Kinase Inhibitors Resulted in Differentiation of hESCs.

Figure 3

(A) Expression of NANOG (Chambers et al., 2003) and OCT4 mRNAs was assessed by RT-PCR, in the presence of protein kinase inhibitors that resulted in differentiation of hESCs. Cells were cultured with 20 ng/ml of bFGF, and inhibitors (10 µM) were included in the cultures for the final 4 days. Inhibitor identities are indicated in the figure. Slower decline of OCT4 than NANOG was typically observed during hESC differentiation. GAPDH was an internal control. (B, C) Undifferentiated, vehicle-only control (B) and differentiated, KDR inhibitor-treated (C) cells are shown under imaging conditions indicated above the columns. All photomicrographs were at the same magnification, and the scale bar (bottom right) represents 50 µM (B, C). Abbreviations: i, inhibitor; uhESCs, undifferentiated hESCs.