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. 2009 Aug 28;5(8):e1000561. doi: 10.1371/journal.ppat.1000561

Figure 5. Entry of PAK into NK cells.

Figure 5

PAK entry into NK cells was evaluated by confocal microscopy and transmission electron microscopy (TEM). NK92 cells were infected with PAK-GFP at MOI of 500 for 8 h. (A) NK cells were detected by staining with CD56-PE (membrane) and DAPI (nuclei); PAK were visualized directly by GFP fluorescence under a confocal microscope (original magnification, 600×). (B) An NK cell with internalized bacteria observed by TEM (original magnification, 3,810×). Inset: bacteria invading via a phagocytic cup formation (closed arrow) and internalized bacteria within a vacuole-like structure (open arrow) (original magnification, 15,000×).