Table 1. Specific elimination of NK cells by P. aeruginosa.
| NK1.1+/CD3e− (NK) | NK1.1+/CD3e+ (NKT) | NK1.1−/CD3e+ (T) | Ly6-G and Ly-6C+ (Gr-1) | CD11b+ (Mac-1) | B220+/CD19+ (B) | Splenocytes | |
| 1×PBS | 7.64±1.43 | 2.39±0.55 | 48.31±14.04 | 32.17±4.38 | 22.34±0.84 | 35.95±4.32 | 265.30±34.69 |
| 2×106 PAK | 5.16±1.38 | 1.99±0.55 | 61.94±9.98 | 38.36±5.98 | 20.65±2.31 | 37.76±6.66 | 277.43±47.88 |
| 2×107 PAK | 1.87±0.58* | 1.43±0.53 | 48.03±10.11 | 28.98±1.76 | 14.62±0.28* | 30.53±8.11 | 230.76±17.09 |
Absolute numbers of immune cells in total splenocytes (×105).
Comparative statistical analyses of immune cell population in spleen at 24 h post infection of PAK were shown in Table 1. The numbers of immune cells including NK cells (NK1.1+/CD3e−), NKT cells (NK1.1+/CD3e+), T cells (NK1.1−/CD3e+), Neutrophils (Ly6G and Ly6C+), Macrophage (CD11b+) and B cells (B220+/CD19+) were determined by calculating the absolute numbers of each cell type from the FACS profiles and total cell numbers in the tissues. Results are expressed as mean±SD of three individual experiments (n = 3, *p<0.05).