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. 2009 Aug 24;4(8):e6720. doi: 10.1371/journal.pone.0006720

Figure 2. Analysis of (p)ppGpp synthesis in Y. pestis KIM6+ and ΔrelA ΔspoT mutants during amino acid and carbon starvation by TLC.

Figure 2

Total intracellular nucleotides were extracted from Y. pestis cultures uniformly labeled with [32P] H3PO4. Cells were grown in modified PMH2 medium lacking L-phenylalanine for amino acid starvation (A) and in modified PMH2 medium without glucose for carbon starvation (B).