Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Evaluation of virulence factor transcription by semi-quantitative RT-PCR. (B) Measurement of secreted virulence factors in culture supernatants by western blotting. Secreted proteins were collected from the culture medium following the removal of bacterial cells. Proteins were separated by SDS-PAGE and detected by western blotting. For each sample, the same amount of total protein was loaded.
