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. 2009 Aug 24;4(8):e6722. doi: 10.1371/journal.pone.0006722

Figure 1. Noggin Treatment Induces Neural Differentiation of Primate Embryonic Stem Cells.

Figure 1

(A) Schematic procedures of a highly efficient motor neuron differentiation system from primate embryonic stem cells are shown. Red text shows medium components and white text in blue boxes shows culture days. (B–G) KhES-1 human or CMK6 cynomolgus monkey ESCs were cultured in the presence of recombinant mouse Noggin for 10 days (Passage 1; P1, B and F) and 7 more days (Passage 2; P2, C and G). Cells were fixed with 4% PFA and stained with antibodies against specific markers: Nestin (NES: neuroectoderm), Desmin (DES: mesoderm), α-fetaprotein (AFP: endoderm), and Oct4 (OCT: undifferentiated ESC) at P1 and P2. Black bar in BF image and white bar in NES image indicate 25 µm (B). Dorsomorphin-induced neural differentiation is shown in D and E.