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(A–D) Spinal motor neuron marker HB9 and rostral neural marker BF1 in the control (A and C) or 1 µM ATRA and 500 ng/ml Shh culture conditions (B and D). (E) HB9 expression was strongly induced by ATRA/Shh treatment while BF1 expression was strongly repressed. *p<0.05 (n = 3).
