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. 2009 Aug 24;4(8):e6722. doi: 10.1371/journal.pone.0006722

Figure 4. ATRA/Shh Induces the Expression of Spinal Cord-Specific and Motor Neuron-Specific Genes.

Figure 4

(A–C) The expression of sMN-specific genes such as HB9, Isl1, and Olig2 was upregulated by the treatment of 1 µM ATRA and 500 ng/ml Shh. (D) A spinal cord-specific gene, HoxB4, was also upregulated in ATRA/SAG-treated cells compared to the control without ATRA/Shh. In contrast, the rostral brain marker BF1 expression was downregulated in ATRA/Shh-treated culture compared to the control culture (E). (F) There was no statistically significant difference in the expression levels of the neuronal marker βIII-tubulin between ATRA/Shh culture and the control. The gene expression levels in the control culture were defined as 1.0.*p<0.05, **p<0.005 (n = 3).