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. 2009 Aug 24;4(8):e6722. doi: 10.1371/journal.pone.0006722

Figure 7. FGF2-treated Neurospheres Produce More sMNs Than Do EGF/FGF2-treated Neurospheres.

Figure 7

(A) Both FGF2- and EGF/FGF2-treated neurospheres passaged though sphere numbers were gradually decreased depending on the number of passages. (B) Neurospheres in two different culture conditions showed no differences in morphology. Black bar indicates 100 µm. (C) The HB9+ sMN differentiation ratio in EGF/FGF2-treated neurospheres decreased more rapidly than in FGF2-treated neurospheres. (D) Immunocytochemistry of differentiated cells derived from EGF/FGF2-treated neurospheres. White bar indicates 20 µm. *p<0.05, **p<0.005 (n = 4).