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| % of HB9+ | % of Recovery | |
| Total | 29.5 | (100)* |
| Interface 1 | 43.6 | 14 |
| Interface 2 | 78.3 | 48 |
| Pellet | 25.6 | 38 |
Separated cells were immunostained with anti-HB9 and βIII-tubulin antibodies.
Recovery ratio of total fraction shows 100%. The recovery ratio was calculated based on numbers of βIII-tubulin-positive cells. The ratio of HB9+ cells was calculated by the following formula: (number of HB9+ cells/number of total cells)×100.
