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. 2009 Aug 25;4(8):e6760. doi: 10.1371/journal.pone.0006760

Figure 6. Subunits of the Ccr4-Not, exosome and TRAMP complexes co-purify.

Figure 6

2 g of total proteins extracted from (A) wild-type cells expressing Tap-tagged Not5 or (B) wild-type and caf40Δ cells expressing Tap-tagged Not4, Not2, or Caf130 as indicated, were subject to tandem affinity purification. After separation on SDS-PAGE, and coomassie staining, the purified proteins were identified by mass spectrometry analysis (see Table S3). The components of the Ccr4-Not, TRAMP or exosome complexes identified are indicated on the right of the gel lanes by numbers which refer to the following proteins: 1 is Not1, 2 is Caf130, 3 is Not3, 4 is Ccr4, 5 is Not5, 6 is Not4, 7 is Caf1, 8 is Caf40, 9 is Not2, 10 is Mtr4, 11 is Rrp41 and 12 is Rrp42. Molecular weight markers (M) are indicated on the left of the gels (in kDa).