Figure 4. Incorporation of VLDL (A) and chylomicrons (B) [³H]triolein into myocardial tissue lipids.

Isolated rat hearts were perfused with very-low-density lipoprotein (VLDL) or chylomicrons (CM) (0.4 mm TAG) prepared from lean control or obese diabetic rats together with [¹⁴C]glucose (11 mm). Lipids were extracted from myocardial tissue and separated by thin-layer chromatography according to lipid class to estimate incorporation of VLDL- or CM-[³H]triolein into tissue [³H]lipid. TAG, triacylglycerol; DAG, diacylglycerol; FA, fatty acid; PL, phospholipid; CE, cholesterol ester. Results are expressed as total mass of [³H]lipid incorporated, and proportion incorporated into each lipid class; for further details see text. Results are expressed as means ±s.e.m. (n= 7 per group). Statistically significant differences between diabetic and control hearts are indicated: *P < 0.05, **P < 0.01; significant differences between diabetic and control lipoproteins are indicated: ^#P < 0.05, ^##P < 0.01; significant differences between diabetic hearts perfused with diabetic lipoproteins and control hearts perfused with control lipoproteins are indicated: ‡P < 0.05.