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. 2009 May 11;587(Pt 13):3317–3328. doi: 10.1113/jphysiol.2009.169300

Figure 5. Effects of oestradiol, ERα and β agonists, and ICI 182,780 on occludin, JAM-A and ZO-1 expression in Caco-2 cells.

Figure 5

The expression of occludin (A), JAM-A (B), and ZO-1 (C) proteins and mRNA were examined by Western blotting (upper panels) and RT-PCR (lower panels), with representative band images and corresponding densitometric analysis. Caco-2 cells were treated for 8 h with ethanol vehicle (C), or with EB (10 nm), DPN (100 nm) or PPT (100 nm), in the presence or absence of ER antagonist ICI 182,780 (ICI, 10 μm). Note that EB like DPN, but not PPT, significantly increased mRNA and protein levels of occludin (A) and JAM-A (B), without effect on ZO-1 expression (C). Both EB and DPN effects on occludin and JAM-A expression were blocked by ICI. Each bar represents mean ±s.e.m. from 6 to 7 independent experiments. P= 0.01, ANOVA; **P < 0.001, *P < 0.05, and ns (not significant) (Bonferroni post hoc test) vs. control cells (C).