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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Anesthesiology. 2009 Jan;110(1):140–149. doi: 10.1097/ALN.0b013e318190bc84

Fig. 4.

Fig. 4

Confocal image of nerve fibers in muscle tissue for nerve growth factor (NGF) and protein gene product 9.5 (PGP 9.5), calcitonin gene related peptide (CGRP), isolectin B4 (IB4) fluorescent immunohistochemistry. A, B and C: NGF fluorescent immunohistochemistry (red) within incised flexor muscle on POD (post operative day) 1. A1: PGP 9.5 immunohistochemistry for nerve axons (green) in incised flexor muscle. A2: Merged image shows NGF colocalizes with PGP9.5 immunoreactive axons (yellow). B1: CGRP immunohistochemistry for axons (green) within incised flexor muscle. B2: Merged image shows NGF colocalizes with CGRP immunoreactive axons (yellow). C1: IB4 labeling (green) in incised flexor muscle. C2: Merged image shows NGF does not colocalize with IB4 axons (yellow).