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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Anesthesiology. 2009 Jan;110(1):140–149. doi: 10.1097/ALN.0b013e318190bc84

Fig. 8.

Fig. 8

In situ hybridization (ISH) for nerve growth factor (NGF) mRNA in incised plantar skin and flexor digitorum brevis muscle. Sections are counter stained with fast red for nuclei. A: ISH for NGF mRNA with the antisense probe 2 hours after incision. B–H: Higher magnification photographs show NGF mRNA expression within epidermis and dermis (B), dense connective tissue in subcutaneous region (C), perimysium (D), peri-vascular connective tissue (E) and large nerve bundle (F) in deep tissue layer. ISH in subcutaneous region adjacent to skin incision (G), and flexor muscle fibers adjacent to muscle incision (H). I: ISH for NGF mRNA with sense probe at 2 hours after incision showing no positive staining. The arrows indicate the incision. PO – post operative.