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. Author manuscript; available in PMC: 2009 Aug 14.
Published in final edited form as: Inorg Chem. 2008 Apr 1;47(9):3480–3482. doi: 10.1021/ic800148a

Figure 2.

Figure 2

UV-vis spectra of the hHO-1/H25A heme complex alone (400 nm) and in the presence of PhSH (388 nm). No CO-bound form was observed after the solution was flushed with CO. The thiolatehHO-1/H25A heme complex was prepared by adding PhSH (~4 equivalents) in ethanol to a 1 mL solution of the hHO-1/H25A heme complex (~5 μM) in 100 mM phosphate (pH 7.4) at 20 °C.