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Comparison of the UV-vis spectrum of the hHO-1/H25A-PhSeH heme complex with that of the ferrous hHO-1/H25A heme complex. The ferrous hHO-1/H25A heme complex was prepared by adding sodium dithionite (SDT, 1 mM) into a 1 mL solution of the hHO-1/H25A heme complex (~5 μM) in 100 mM phosphate (pH 7.4) at 20 °C under anaerobic conditions.
