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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Neurochem. 2009 May;109(3):923–934. doi: 10.1111/j.1471-4159.2009.06021.x

FIGURE 3. Effects of replacement of the conserved TM1 tyrosine residue with alanine on the potency of agonists for P2XRs.

FIGURE 3

Concentration response curves to agonists in WT (closed circles) and TM1 tyrosine to alanine mutants (open circles) of P2XRs expressed in HEK293 cells. (Left) Increase in the sensitivity of the P2X2R (A), P2X3R (B), and P2X4R (C) mutants to ATP and the lack of effects of TM1-Tyr mutation on the sensitivity of P2X7R receptors to BzATP (D). (Right) A leftward shift in the sensitivity of TM1-Tyr mutants of P2X2R (A), P2X3R (B), and P2X4R (C) to αβ-meATP and the lack of effects of this mutation on the sensitivity of P2X7R to ATP (D). Vertical dashed lines indicate the EC50 value, and mean±SEM values are shown in Table 2. Each concentration was examined in between five and 30 different cells and current responses were normalized to an averaged maximum current induced by 100 µM ATP for the P2X2R, P2X3R, and P2X4R and 300 µM BzATP for the P2X7R.