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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Neurochem. 2009 May;109(3):923–934. doi: 10.1111/j.1471-4159.2009.06021.x

FIGURE 4. Partial rescue of receptor function by replacement of the P2X4R-Tyr42 residue with phenylalanine and tryptophan.

FIGURE 4

(A) Effects of replacement of the P2X4R-Tyr42 residue with alanine, isoleucine, cysteine, glycine, phenylalanine, tryptophan, aspartate, or glutamate on the rates of receptor deactivation. Superimposed responses to ATP (0.3–1 µM) of WT and functional mutants are shown. The order of the deactivation time constants for current decay after ATP removal is shown below the traces. (B) Logarithmic relationship between the EC50 values for ATP and deactivation time constants for functional P2X4R-Tyr42 mutants. (C) The expression pattern of non-functional EGFP-tagged P2X4-Y42D and P2X4-Y42R mutants in HEK293 cells.