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Plate-attached synaptosomes and mitochondria distribute evenly on the bottom of the microplate wells. (A) Cortical synaptosomes plated at 10 μg protein/well or (B) isolated liver mitochondria plated at 2.5 μg protein/well were stained with Mitotracker Red, and the bottom of the well was imaged with fluorescence microscopy. (a) The round structures are the spacers (Figure 1A and B (g)) which set the height of the chamber.
