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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Transl Res. 2009 May 9;154(1):18–26. doi: 10.1016/j.trsl.2009.04.003

Figure 3. Adiponectin isoforms found in the urine and plasma of patients with SLE nephritis.

Figure 3

Urine or plasma proteins from 3 healthy individuals and 6 patients with lupus nephritis were separated on non-reducing, non-denaturing gels to preserve native protein structure, and were immunoblotted for adiponectin. The high, middle (hexamer), and low molecular weight adiponectin isoforms are labeled HMW, MMW, LMW, respectively. In panel 1, CON indicates a urine sample from a healthy individual (representative of 3), and AD represents the adiponectin isoforms produced by human fat as a reference. Panel 2 shows urine adiponectin isoforms during a lupus nephritis flare cycle. The samples were from four and two months before flare (PRE), at renal flare (F), and 4 months after flare (POST). Panel 3 shows plasma (P) and urine (U) adiponectin isoforms from two lupus patients during renal flare (patient 1 lanes 1, 2; patient 2 lanes 5, 6). Lanes 3 and 4 are from a third SLE patient 10 months after a renal flare was diagnosed. The patient was improving but still had over 5 grams of urine protein/day. Panel 4 shows the plasma (P) and urine (U) of two patients blotted for adiponectin in the absence and presence of human recombinant adiponectin (rAdip).